Improved methods are needed for routine, inexpensive monitoring of biomarkers that could facilitate earlier detection and characterization of cancer. Suspended microchannel resonators (SMRs) are highly sensitive, batch-fabricated microcantilevers with embedded microchannels that can directly quantify adsorbed mass via changes in resonant frequency. As in other label-free detection methods, biomolecular measurements in complex media such as serum are challenging due to high background signals from nonspecific binding. In this report, we demonstrate that carboxybetaine-derived polymers developed to adsorb directly onto SMR SiO[subscript 2] surfaces act as ultralow fouling and functionalizable surface coatings. Coupled with a reference microcantilever, this approach enables detection of activated leukocyte cell adhesion molecule (ALCAM), a model cancer biomarker, in undiluted serum with a limit of detection of 10 ng/mL.
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机译：需要对生物标志物进行常规，廉价监测的改进方法，以利于早期检测和鉴定癌症。悬挂式微通道谐振器（SMR）是具有嵌入式微通道的高灵敏度，批量制造的微悬臂，可通过谐振频率的变化直接量化吸附质量。与其他无标记检测方法一样，由于来自非特异性结合的高背景信号，在复杂介质（如血清）中进行生物分子测量也具有挑战性。在此报告中，我们证明了开发来直接吸附到SMR SiO [subscript 2]表面上的羧基甜菜碱衍生的聚合物可作为超低结垢和可官能化的表面涂层。结合参考微悬臂梁，此方法可在未稀释的血清中检测活化的白细胞粘附分子（ALCAM）（一种模型癌症生物标记物），检测极限为10 ng / mL。
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